Shaping Durum Wheat for the Future: Gene Expression Analyses and Metabolites Profiling Support the Contribution of BCAT Genes to Drought Stress Response
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Shaping Durum Wheat for the Future: Gene Expression Analyses and Metabolites Profiling Support the Contribution of BCAT Genes to Drought Stress Response
global climate change, its implications for agriculture, and complex scenarios presented by the scientific community to the attention of the whole world. Drought is a major abiotic stress which can limit growth and yield, so the identification of genotypes with a higher adaptation to drought is one of the major objectives in breeding programs. During abiotic stress, metabolic adaptation is very important for stress tolerance, and specific accumulation of amino acids and / or as secondary metabolites derived from the metabolism of amino acids can be correlated with increased tolerance to adverse environmental conditions.
This work, focused on the metabolism of branched chain amino acids (BCAA) in durum wheat and the role of branched-chain amino acid aminotransferase (BCATs) in response to stress. BCATs role in response to drought crops previously proposed for Arabidopsis, in which the BCAA levels are altered in the level of transcription in drought conditions, lead to metabolic defense response. However, the role of BCAA wheat as drought triggers defense responses have not been described. Comparative genomic approaches described composition BCAT gene family in durum wheat.
Here we show the tissue and developmental stage specificity BCATs regulation in response to drought. In addition, metabolite profiling was performed on two contrasting cultivars of durum wheat Cappelli Colosseo and resulted in detection of a specific pattern of metabolites accumulated between genotype and, in particular, in enhanced BCAA accumulated in Cappelli tolerant cv further supports the role of BCAAs in defense responses to drought. The results support the use of gene expression and metabolomics in modern breeding objectives to form new cultivars that are more resistant to climate change.
Shaping Durum Wheat for the Future: Gene Expression Analyses and Metabolites Profiling Support the Contribution of BCAT Genes to Drought Stress Response
Gene expression profiles of cardiomyocytes hypertrophic identify new players in the pathological remodeling
Objective: pathological cardiac remodeling characterized by cardiomyocyte hypertrophy and activation of fibroblasts, which in turn can lead to maladaptive hypertrophy and heart failure (HF). Genome-wide expression analysis in cardiac tissue has been instrumental to the identification of molecular mechanisms play. However, the data is based on signals originating from all types of heart cells. Here we are aiming for a more detailed view of the molecular changes driving maladaptive cardiomyocyte hypertrophy to aid in the development of therapies to reverse the pathological remodeling.
Methods and results: Utilizing special cardiomyocyte reporter mice exposed to pressure overload by aortic cross-appeal and cardiomyocyte isolation by flow cytometry, we obtained gene expression profiles of hypertrophic cardiomyocytes in a faster pace after the stress and pathological hypertrophy of cardiomyocytes show. We identified a subset of genes differentially regulated and specific to either stage.
Among the specific genes upregulated in cardiomyocytes during maladaptive phase we found a stress marker is known, as Nppb and Myh7, but also identified a set of genes with unknown role in pathological hypertrophy, including platelet isoform of phosphofructokinase (PFKP). norepinephrine-angiotensin II treatment of cultured human cardiomyocytes due to the secretion of NT pro-BNP and recapitulation upregulation of this gene, suggesting conservation failed upregulation in cardiomyocytes. In addition, several genes induced during pathological hypertrophy was also found elevated in human heart failure, with their expression was positively correlated with stress markers known NPPB and MYH7.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ABIN3 . This antibody is tested and proven to work in the following applications:
Description: ABIN3 Antibody: The nuclear factor NF-κB plays key roles in development and immunity. ABIN3 (A20-binding inhibitor of NF-κB activation 3), also known as TNFAIP3-interacting protein 3 (TNIP3), is a novel negative feedback regulator of LPS-induced NF-κB activation. ABIN3 is a 39 kDa protein that negatively regulates NF-κB activation in response to TNF and LPS. ABIN3 is highly expressed in brain, thymus, lymph node, lung and fetal liver, with low expression in kidney, bone marrow. Through its interaction with A20, ABIN3 interferes with TRAF2-mediated transactivation signals and NF-κB inhibition is mediated by the ABIN-homology domain 2. ABIN3 has been found to be induced by Listeria infection and can be slightly downregulated by dexamethasone. Enhanced expression of ABIN3 in monocytes is associated with sepsis. Thus, ABIN3 is an IL-10-induced gene product capable of attenuating NF-κB in human macrophages yet is inoperative in mice and represents a basis for species-specific differences in IL-10 actions. At least four isoforms of ABIN3 are known to exist.
Description: ABIN3 Antibody: The nuclear factor NF-κB plays key roles in development and immunity. ABIN3 (A20-binding inhibitor of NF-κB activation 3), also known as TNFAIP3-interacting protein 3 (TNIP3), is a novel negative feedback regulator of LPS-induced NF-κB activation. ABIN3 is a 39 kDa protein that negatively regulates NF-κB activation in response to TNF and LPS. ABIN3 is highly expressed in brain, thymus, lymph node, lung and fetal liver, with low expression in kidney, bone marrow. Through its interaction with A20, ABIN3 interferes with TRAF2-mediated transactivation signals and NF-κB inhibition is mediated by the ABIN-homology domain 2. ABIN3 has been found to be induced by Listeria infection and can be slightly downregulated by dexamethasone. Enhanced expression of ABIN3 in monocytes is associated with sepsis. Thus, ABIN3 is an IL-10-induced gene product capable of attenuating NF-κB in human macrophages yet is inoperative in mice and represents a basis for species-specific differences in IL-10 actions. At least four isoforms of ABIN3 are known to exist.
Description: A polyclonal antibody for detection of Abin-2 from Human, Mouse, Rat. This Abin-2 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human Abin-2 protein at amino acid sequence of 61-110
Description: A polyclonal antibody for detection of Abin-2 from Human, Mouse, Rat. This Abin-2 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human Abin-2 protein at amino acid sequence of 61-110
Description: A polyclonal antibody for detection of Abin-2 from Human, Mouse, Rat. This Abin-2 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human Abin-2 protein at amino acid sequence of 61-110
Description: This gene encodes a protein which acts as an inhibitor of NFkappaB activation. The encoded protein is also involved in MAP/ERK signaling pathway in specific cell types. It may be involved in apoptosis of endothelial cells. Alternative splicing results in multiple transcript variants. A pseudogene related to this gene is located on the X chromosome.
Description: This gene encodes a protein which acts as an inhibitor of NFkappaB activation. The encoded protein is also involved in MAP/ERK signaling pathway in specific cell types. It may be involved in apoptosis of endothelial cells. Alternative splicing results in multiple transcript variants. A pseudogene related to this gene is located on the X chromosome.
Description: This gene encodes a protein which acts as an inhibitor of NFkappaB activation. The encoded protein is also involved in MAP/ERK signaling pathway in specific cell types. It may be involved in apoptosis of endothelial cells. Alternative splicing results in multiple transcript variants. A pseudogene related to this gene is located on the X chromosome.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TNIP2 / ABIN-2 . This antibody is tested and proven to work in the following applications:
Elongation Factor 1A2 Binding Protein Rabbit Polyclonal Antibody
Mechanically, the suppression Pfkp in primary cardiomyocytes attenuated stress gene expression and hypertrophy, suggesting that Pfkp an important new player in the renovation of pathological cardiomyocyte.
