Predicting brain metastasis in early stage non-small cell lung cancer patients by gene expression profiling
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Predicting brain metastasis in early stage non-small cell lung cancer patients by gene expression profiling
Background: Non-small cell lung cancer (NSCLC) is the most common cause of cancer-deaths due to early metastatic spread, in many cases, especially to the brain. The pattern of the spread of disease-specific organ may be driven by the activity of specific signaling pathway in the primary tumor. We aim to identify gene expression signatures and the relevant signal associated with the development of brain metastasis (BM) after surgical resection of NSCLC.
Methods: frozen Fast NSCLC surgical specimens obtained from the tumor bank. RNA was extracted and analyzed by RNA-sequencing (Illumina HiSeq 2500). clinical parameters and gene expression examined to distinguish between patients with BM, patients with metastasis to sites other than the brain, and patients who do not develop metastatic disease in a significant clinical follow-up. Study component analysis and principal enrichment lines do.
Results: A total of 91 patients were included in this study, 32 of which were developed BM. The stage of disease at diagnosis (P = 0.004) and the degree of differentiation (P = 0.007) were significantly different between BM and control groups. We identified a set of 22 genes that are associated specifically with the BM, and not with metastasis to other sites. This sets reached 93.4% accuracy (95% CI: 86.2 to 97.5%), 96.6% specificity and 87.5% sensitivity of identifying the correct patient BM in the analysis of the internal validation leave-one-out , The oxidative phosphorylation pathway was highly correlated with the risk of BM.
Conclusion: The expression level of a small set of genes from the primary tumor was found to predict the development of BM, clear of metastasis to other organs. Oxidative phosphorylation correlated genes and pathways require further validation as a useful predictor of the clinical potential of BM and perhaps as a new therapeutic target for the prevention of BM.
Predicting brain metastasis in early stage non-small cell lung cancer patients by gene expression profiling
Gene expression profile identifies Zac1 role in metastatic cervical cancer
Zinc-finger proteins that regulate apoptosis and cell cycle arrest 1 (Zac1), encoded by the gene Plagl1, is a transcription factor of seven zinc-finger-containing belong printed genome and is expressed in various types of human embryonic and adult tissue. Zac1 is postulated be a tumor suppressor by inducing cell cycle arrest and apoptosis through interacting and modulating the transcriptional activity of p53 as named.
Correspondingly, reduction or loss of expression Zac1 associated with the occurrence and development of some human tumors, including cervical cancer, breast cancer, ovarian cancer, pituitary tumors and basal cell carcinoma, implying rationality of utilizing the expression Zac1 as a biomarker novel for evaluating the prognosis of cervical cancer.
However, until now, has not been clarified whether Zac1 expression associated with the prognosis of patients in clinical cervical cancer tumor samples. To answer the questions outlined above, we report here a comprehensive investigation Zac1 expression in biopsies of cervical carcinoma clinical. By analyzing the expression Zac1 in a variety of gene expression profiling of cervical cancer database, we demonstrated an association between high Zac1 expression and poor prognosis of cervical cancer.
Description: Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF-dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway.
Description: Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF-dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway.
Description: Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF-dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway.
Description: Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF-dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway.
Description: Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF-dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway.
Description: Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF-dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway.
Description: Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF-dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway.
Description: Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF-dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway.
Description: Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF-dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway.
Description: Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF-dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway.
Description: Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF-dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human RUSC1 . This antibody is tested and proven to work in the following applications:
Description: RUSC1 Antibody: RUSC1, also known as NESCA, shares with the related protein RUSC2 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% amino acid identity. RUSC1 is an adapter protein that can bind to the TrkA receptor and is necessary in the NGF-induced neurite growth of PC12 cells. RUSC1 has also been shown to interact with IκB kinase- (IKK-) gamma, the regulatory subunit of the IKK complex that is required for NF-κB activation in many signaling pathways such as TNF-R or the TLR pathways. RUSC1 can also bind to the E3 ubiquitin ligase TRAF6, which then catalyzes RUSC1 polyubiquitination. Since overexpression of RUSC1 strongly inhibits TRAF6-mediated polyubiquitination of IKK-gamma, RUSC1 may be a link in the IKK-gamma-mediated NF-κB activation pathway.
Description: RUSC1 Antibody: RUSC1, also known as NESCA, shares with the related protein RUSC2 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% amino acid identity. RUSC1 is an adapter protein that can bind to the TrkA receptor and is necessary in the NGF-induced neurite growth of PC12 cells. RUSC1 has also been shown to interact with IκB kinase- (IKK-) gamma, the regulatory subunit of the IKK complex that is required for NF-κB activation in many signaling pathways such as TNF-R or the TLR pathways. RUSC1 can also bind to the E3 ubiquitin ligase TRAF6, which then catalyzes RUSC1 polyubiquitination. Since overexpression of RUSC1 strongly inhibits TRAF6-mediated polyubiquitination of IKK-gamma, RUSC1 may be a link in the IKK-gamma-mediated NF-κB activation pathway.
Description: A polyclonal antibody against RUSC1. Recognizes RUSC1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200
Description: A polyclonal antibody against RUSC1. Recognizes RUSC1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC
Functional enrichment analysis showed that expression of high Zac1 associated with epithelial-mesenchymal transition (EMT), which was subsequently observed in clinical characteristics and metastatic carcinoma samples using immunohistochemical staining. Correspondingly, hypomethylation CpG islands in the promoter Zac1 observed in samples with high Zac1 expression in cervical carcinoma.
