Molecular characterisation, tissue distribution, and expression profiling of the cathepsin b gene during ovarian follicle development in geese
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Molecular characterisation, tissue distribution, and expression profiling of the cathepsin b gene during ovarian follicle development in geese
Although there is evidence that cathepsin B (CTSB) regulates the degradation and absorption of yellow precursors during the development of the bird ovarian follicle, nothing is known about its molecular characteristics, its tissue distribution profiles or expressions in ovarian ovarian follicular compartments. The intact 1023 bp coding sequence of the GOOSE CTSB gene was obtained for the first time. It has coded a polypeptide of 340 amino acids (AA) containing two conserved functional domains (i.e. propece_c1 and peptidase_c1a_cathps b) and three active amino acid residues (+108, +279 and +299). The nucleotide and AA sequences of GOOSE CTSB gene showed more than 90% similarity with its respective counterparts of other avian species. The results of the QRT PCR showed that the CTSB mRNA was ubiquitously expressed in all goose tissues examined, with moderate high levels in reproductive organs, including stroma and oviducat oviducat.
The expression of the CTSB OIE mRNA in the granulosa layers gradually increased Follicles F5 of 2-4 mm but decreased at relatively low levels in Follicles F4-F1, while remaining statistically unchanged unchanged in The layers of the theca in the development of the follicle. Similar high sequence of the GOOSE CTSB gene to other avian species suggested functional preservation of bird CTSB genes and its fluctuation levels in granulosa layers can be associated with the orderly progression of Folliciennes Goose. These data asked a base for elucidating the role of CTSB in the bird ovary. We areolate the MCECs exposed to both glucose conditions.
The MCCs showed well-organized tubes and a dynamic migration in the NG condition, while the formation of tubes and migration were considerably reduced in the HG condition. The NRA sequencing analysis showed that MCEC had different gene profiles under NG and HG conditions. Among the significantly modified genes, we have ranked 14 categories of major genes, we have identified as aging genes (9.22%) and angiogenesis (9.06%) have changed the most. Thirteen genes of the two gene categories showed constant modifications on the NRA sequencing test, and these results were validated by RT-PCR.
Profiling expression of sexually dimorphic genes in the Japanese quail, Coturnix Japonica
Research on the determination of avian sex has focused on chicken. In this study, we have established the utility of another widely used animal model, the Japanese quail (Coturnix Japonica), to clarify the molecular mechanisms underlying the gonadal sexual differentiation. In particular, we performed a full gene expression of three-step embryonic gonads. We have classified the expression schemes of 4,815 genes in nine clusters depending on the size of the change between the steps.
Cluster 2 (characterized by an initial increase and levels stable thereafter), including 495 and 310 genes expressed in men and women, respectively, contained five key genes involved in gonadal sexual differentiation. A GO analysis showed that the genes of this group are linked to development processes, including the development of the reproductive structure and development processes involved in reproduction, suggesting that the expression profile is an effective approach to Identify new candidate genes. Based on RNA-SEQ data and in situ hybridization, expression models and location of most main genes for gonadal sexual differentiation corresponded to those of the chicken. Our results support the effectiveness of the Japanese quail as a model of gonadal sexual differentiation studies in birds.
Molecular characterisation, tissue distribution, and expression profiling of the cathepsin b gene during ovarian follicle development in geese
Genic expression Profiling of human neutrophils after infection with acinetobacter baumannii in vitro
Acinetobacter Baumannii is a nosocomial pathogen with grams that has gained growing global reputation because of its wide range of antibiotic resistance and mortality rates in hospitalized patients. Therefore, it is necessary to better understand the key aspects of pathogenesis A. baumannii, such as host-pathogenic interactions. In this report, we analyzed both a gene expression and the production of cytokines by human neutrophils infected with A. Baumannii. Our tests reveal a proximal response of neutrophils after A. Baumannii infection, since the intracellular transcription of the effector proteins such as COX-2, transcription factors and preflammatory cytokines have significantly resulted in neutrophils infected with neutrophils of A. Baumannii. The translation and release of CXCL-8, IL-1β and TNF-α by neutrophils have been confirmed by quantification of proteins in culture supernators.
The results obtained in this report reinforce the importance of human neutrophils in the control of infections A. Baumannii, but also highlight the proximal nature of these host-pathogenic interactions as target for future immunomodulatory therapies. Axons are vulnerable to injury, which potentially causes degeneration or neuronal death. Although the neurons of the central nervous system do not regenerate, the neurons of the peripheral nervous system are known to regenerate. As shown that the transduction of the injury response signal is mediated by genes expression changes, expression profiling is a useful tool for understanding the molecular mechanisms of regeneration.
Description: Fetal human uterus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human uterus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the uterus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The uterus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Description: Human uterus-corpus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-corpus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-corpus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-corpus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Description: Human uterus-fundus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-fundus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-fundus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-fundus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
The regeneration of Axon is governed by genes sensitive to injuries induced in both neurons and their surrounding non-neuronal cells. Thus, an experimental configuration for the comparative analysis between the regeneration and non-regenerative conditions is essential to identify ideal objectives for the promotion of regeneration-related genes and understand the mechanisms of Axon regeneration. Here we look at the original research that indicates the key factors governing Axon regeneration, including using the expression profile of comparative genes in various systems.
