Molecular characterisation, tissue distribution, and expression profiling of the cathepsin b gene during ovarian follicle development in geese

Although there is evidence that cathepsin B (CTSB) regulates the degradation and absorption of yellow precursors during the development of the bird ovarian follicle, nothing is known about its molecular characteristics, its tissue distribution profiles or expressions in ovarian ovarian follicular compartments. The intact 1023 bp coding sequence of the GOOSE CTSB gene was obtained for the first time. It has coded a polypeptide of 340 amino acids (AA) containing two conserved functional domains (i.e. propece_c1 and peptidase_c1a_cathps b) and three active amino acid residues (+108, +279 and +299). The nucleotide and AA sequences of GOOSE CTSB gene showed more than 90% similarity with its respective counterparts of other avian species. The results of the QRT PCR showed that the CTSB mRNA was ubiquitously expressed in all goose tissues examined, with moderate high levels in reproductive organs, including stroma and oviducat oviducat.

The expression of the CTSB OIE mRNA in the granulosa layers gradually increased Follicles F5 of 2-4 mm but decreased at relatively low levels in Follicles F4-F1, while remaining statistically unchanged unchanged in The layers of the theca in the development of the follicle. Similar high sequence of the GOOSE CTSB gene to other avian species suggested functional preservation of bird CTSB genes and its fluctuation levels in granulosa layers can be associated with the orderly progression of Folliciennes Goose. These data asked a base for elucidating the role of CTSB in the bird ovary. We areolate the MCECs exposed to both glucose conditions.

The MCCs showed well-organized tubes and a dynamic migration in the NG condition, while the formation of tubes and migration were considerably reduced in the HG condition. The NRA sequencing analysis showed that MCEC had different gene profiles under NG and HG conditions. Among the significantly modified genes, we have ranked 14 categories of major genes, we have identified as aging genes (9.22%) and angiogenesis (9.06%) have changed the most. Thirteen genes of the two gene categories showed constant modifications on the NRA sequencing test, and these results were validated by RT-PCR.

Profiling expression of sexually dimorphic genes in the Japanese quail, Coturnix Japonica

Research on the determination of avian sex has focused on chicken. In this study, we have established the utility of another widely used animal model, the Japanese quail (Coturnix Japonica), to clarify the molecular mechanisms underlying the gonadal sexual differentiation. In particular, we performed a full gene expression of three-step embryonic gonads. We have classified the expression schemes of 4,815 genes in nine clusters depending on the size of the change between the steps.

Cluster 2 (characterized by an initial increase and levels stable thereafter), including 495 and 310 genes expressed in men and women, respectively, contained five key genes involved in gonadal sexual differentiation. A GO analysis showed that the genes of this group are linked to development processes, including the development of the reproductive structure and development processes involved in reproduction, suggesting that the expression profile is an effective approach to Identify new candidate genes. Based on RNA-SEQ data and in situ hybridization, expression models and location of most main genes for gonadal sexual differentiation corresponded to those of the chicken. Our results support the effectiveness of the Japanese quail as a model of gonadal sexual differentiation studies in birds.

 Molecular characterisation, tissue distribution, and expression profiling of the cathepsin b gene during ovarian follicle development in geese
Molecular characterisation, tissue distribution, and expression profiling of the cathepsin b gene during ovarian follicle development in geese

Genic expression Profiling of human neutrophils after infection with acinetobacter baumannii in vitro

Acinetobacter Baumannii is a nosocomial pathogen with grams that has gained growing global reputation because of its wide range of antibiotic resistance and mortality rates in hospitalized patients. Therefore, it is necessary to better understand the key aspects of pathogenesis A. baumannii, such as host-pathogenic interactions. In this report, we analyzed both a gene expression and the production of cytokines by human neutrophils infected with A. Baumannii. Our tests reveal a proximal response of neutrophils after A. Baumannii infection, since the intracellular transcription of the effector proteins such as COX-2, transcription factors and preflammatory cytokines have significantly resulted in neutrophils infected with neutrophils of A. Baumannii. The translation and release of CXCL-8, IL-1β and TNF-α by neutrophils have been confirmed by quantification of proteins in culture supernators.

The results obtained in this report reinforce the importance of human neutrophils in the control of infections A. Baumannii, but also highlight the proximal nature of these host-pathogenic interactions as target for future immunomodulatory therapies. Axons are vulnerable to injury, which potentially causes degeneration or neuronal death. Although the neurons of the central nervous system do not regenerate, the neurons of the peripheral nervous system are known to regenerate. As shown that the transduction of the injury response signal is mediated by genes expression changes, expression profiling is a useful tool for understanding the molecular mechanisms of regeneration.

Mouse CD1 Uterus, non-pregnant Total RNA

MR-411 0.1mg
EUR 160

Mouse C57 Uterus, non-pregnant Total RNA

MR-411-C57 0.1mg
EUR 180

Mouse Balbc Uterus, non-pregnant Total RNA

MR-411-BLC 0.1mg
EUR 180

Mouse CD1 Uterus, non-pregnant Total Protein

MT-411 0.5mg
EUR 153

Mouse BLC Uterus, non-pregnant Total Protein

MT-411-BLC 0.5mg
EUR 180

Mouse C57 Uterus, non-pregnant Total Protein

MT-411-C57 0.5mg
EUR 180

Dog Uterus RNA*

DR-411 0.05mg
EUR 195

Cat Uterus cDNA

FD-411 30 Reactions
EUR 319

Dog Uterus cDNA*

DD-411 30 Reactions
EUR 319

Pig Uterus cDNA

PD-411 30 reactions
EUR 280

Mouse CD1 Uterus, non-pregnant Frozen Sections

MF-411 10 slides
EUR 228

Mouse BLC Uterus, non-pregnant Frozen Sections

MF-411-BLC 10 slides
EUR 253

Mouse C57 Uterus, non-pregnant Frozen Sections

MF-411-C57 10 slides
EUR 253

Total RNA - Human Adult Normal Tissue: Uterus: Cervix of uterus

R1234275-50 50 ug
EUR 221

Total RNA - Human Adult Normal Tissue: Uterus: Corpus of Uterus

R1234276-10 10 ug
EUR 229

Sheep Uterus cDNA

SD-411 30 reactions
EUR 243

Mouse CD1 Uterus, non-pregnant Paraffin Sections

MP-411 10 slides
EUR 228

Mouse BLC Uterus, non-pregnant Paraffin Sections

MP-411-BLC 10 slides
EUR 253

Mouse C57 Uterus, non-pregnant Paraffin Sections

MP-411-C57 10 slides
EUR 253

Bovine Uterus cDNA

BD-411 30 reactions
EUR 243

Rabbit Uterus cDNA

TD-411 30 reactions
EUR 243

Uterus Tissue block

29 1 unit
EUR 535

Hamster Uterus cDNA

AD-411 30 reactions
EUR 243

BOVINE, UTERUS, FRESH

8600861 1EA
EUR 67.09

MiniPig Uterus cDNA

ND-411 30 reactions
EUR 358

Fetal Uterus Lysate

XBL-10431 0.1 mg
EUR 632.4
Description: Fetal human uterus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human uterus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the uterus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The uterus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

PORCINE, UTERUS, FRESH

8604961 1EA
EUR 65.63

BOVINE, UTERUS, FROZEN

8620861 1EA
EUR 67.09

PORCINE, UTERUS, FROZEN

8624961 1EA
EUR 65.63

Rat Uterus Fibroblasts

ABC-TC4237 1 vial Ask for price
Description: Rat uterus fibroblasts, 6-week Wistar rat

OORA00351-10U - UTERUS

OORA00351-10U 1Each
EUR 105

OORA00351-1EA - UTERUS

OORA00351-1EA 1Each
EUR 99

OORA00493-10U - UTERUS

OORA00493-10U 1Each
EUR 55

OORA00493-1EA - UTERUS

OORA00493-1EA 1Each
EUR 59

Uterus Membrane Lysate

XBL-11023 0.1 mg
EUR 619.8
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Mouse C57 Uterus, non-pregnant cDNA-Random Primer

MD-411-C57-RH 30 reactions
EUR 280

Mouse CD1 Uterus, non-pregnant cDNA-Random Primer

MD-411-HR 30 reactions
EUR 243

Cat Uterus Total RNA*

FR-411 0.05mg
EUR 195

Pig Uterus Total RNA

PR-411 0.1mg
EUR 235

Cat Uterus Genomic DNA

FG-411 0.1mg
EUR 210

Dog Uterus Genomic DNA

DG-411 0.1mg
EUR 210

Guinea Pig Uterus cDNA

GD-411 30 reactions
EUR 243

Rat Uterus Genomic DNA

RG-411 0.1mg
EUR 177

Sheep Uterus Total RNA

SR-411 0.1mg
EUR 160

Total RNA - Lupus: Uterus

R1236274Lup-50 50 ug
EUR 460

Pig Uterus Genomic DNA

PG-411 0.1mg
EUR 177

Bovine Uterus Total RNA

BR-411 0.1mg
EUR 160

Rabbit Uterus Total RNA

TR-411 0.1mg
EUR 160

Mouse Normal Uterus (5 slides/pack, single section/slide)

S-MoFPT046 1 unit
EUR 95

Dog Uterus Total Protein

DT-411 1mg
EUR 176

Cat Uterus Total Protein

FT-411 1mg
EUR 176

Hamster Uterus Total RNA*

AR-411 0.05mg
EUR 160

Human Uterus Genomic DNA

HG-411 0.05mg
EUR 210

MiniPig Uterus Total RNA

NR-411 0.1mg
EUR 231

Pig Uterus Total Protein

PT-411 1mg
EUR 153

Sheep Uterus Genomic DNA

SG-411 0.1mg
EUR 177

Total RNA - Human Adult Normal Tissue 5 Donor Pool: Uterus: Cervix of uterus

R1234275-P 50 ug
EUR 443

Bovine Uterus Genomic DNA

BG-411 0.1mg
EUR 177

Equine Uterus Genomic DNA

GE-411 0.1mg
EUR 210

Rabbit Uterus Genomic DNA

TG-411 0.1mg
EUR 177

Human Uterus Tumor lysate

HTL-1384 1 mg
EUR 927.6

OORA00552-1U - Rat UTERUS

OORA00552-1U 1Each
EUR 60

Uterus tumor tissue array

UT802a each
EUR 306
Description: Uterus tumor tissue array, including stromal and leiomyo sarcoma, endometrioid adenocarcinoma, chorionepithelioma, hydatidiform mole, clear cell carcinoma, carcinsarcoma, sarcomatoid carcinoma, with pathology grade, TNM/Stage (AJCC 8th edition), 80 cases/80 cores (cores size 1.5mm), replacing UT802

Uterus Tissue Slide (Benign)

11-402-10um 10 um
EUR 241.8

Uterus Tissue Slide (Benign)

11-402-4um 4 um
EUR 216.6

Uterus Tissue Slide (Normal)

11-401-10um 10 um
EUR 241.8

Uterus Tissue Slide (Normal)

11-401-4um 4 um
EUR 216.6

Hamster Uterus Genomic DNA

GA-411 0.1mg
EUR 177

Chicken Uterus Genomic DNA

GC-411 0.1mg
EUR 177

Sheep Uterus Total Protein

ST-411 1mg
EUR 153

Human Uterus Tissue Lysate

IHUUTETL100UG each
EUR 245
Description: Human Uterus Tissue Lysate

OORA00552-1EA - Rat UTERUS

OORA00552-1EA 1Each
EUR 59

Pig Uterus Frozen Sections

PF-411 10 slides
EUR 261

57153-2 BV UTERUS 5 EA*

57153-2 5 EA
EUR 300

Bovine Uterus Total Protein

BT-411 1mg
EUR 153

Rabbit Uterus Total Protein

TT-411 1mg
EUR 153

Uterus Tissue Slide (Abnormal)

11-419-10um 10 um
EUR 241.8

Uterus Tissue Slide (Abnormal)

11-419-4um 4 um
EUR 216.6

Chicken Uterus Total Protein

CT-411 1mg
EUR 140

Hamster Uterus Total Protein

AT-411 1mg
EUR 153

OORA00523-1U - Rabbit UTERUS

OORA00523-1U 1Each
EUR 255

MiniPig Uterus Total Protein

NT-411 1mg
EUR 176

Pig Uterus Paraffin Sections

PP-411 10 slides
EUR 240

Sheep Uterus Frozen Sections

SF-411 10 slides
EUR 261

Uterus Membrane Tumor Lysate

XBL-11031 0.1 mg
EUR 752.1
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Equine Uterus Frozen Sections

EF-411 10 slides
EUR 261

Bovine Uterus Frozen Sections

BF-411 10 slides
EUR 261

Rabbit Uterus Frozen Sections

TF-411 10 slides
EUR 240

OORA00523-1EA - Rabbit UTERUS

OORA00523-1EA 1Each
EUR 239

Uterus Tissue Slide (Adenomyoma)

11-432-10um 10 um
EUR 241.8

Uterus Tissue Slide (Adenomyoma)

11-432-4um 4 um
EUR 216.6

Hamster Uterus Frozen Sections

AF-411 10 slides
EUR 240

Sheep Uterus Paraffin Sections

SP-411 10 slides
EUR 240

Human Uterus Paraffin Sections

HP-411 10 slides
EUR 319

MiniPig Uterus Frozen Sections

NF-411 10 slides
EUR 307

OORA00470-10U - Hamster UTERUS

OORA00470-10U 1Each
EUR 75

OORA00470-1EA - Hamster UTERUS

OORA00470-1EA 1Each
EUR 69

Rat Uterus-E12 Total RNA

RR-411-12 0.05mg
EUR 160

Rat Uterus-E13 Total RNA

RR-411-13 0.05mg
EUR 160

Rat Uterus-E14 Total RNA

RR-411-14 0.05mg
EUR 160

Rat Uterus-E15 Total RNA

RR-411-15 0.05mg
EUR 160

Rat Uterus-E16 Total RNA

RR-411-16 0.05mg
EUR 160

Rat Uterus-E17 Total RNA

RR-411-17 0.05mg
EUR 160

Rat Uterus-E18 Total RNA

RR-411-18 0.05mg
EUR 160

Rat Uterus-E19 Total RNA

RR-411-19 0.05mg
EUR 160

Rat Uterus-E20 Total RNA

RR-411-20 0.05mg
EUR 160

Equine Uterus Paraffin Sections

EP-411 10 slides
EUR 240

Bovine Uterus Paraffin Sections

BP-411 10 slides
EUR 240

OCPB00133-100UG - Uterus Lysate

OCPB00133-100UG 0.1mg
EUR 139

Uterus-Corpus Membrane Lysate

XBL-11037 0.1 mg
EUR 619.8
Description: Human uterus-corpus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-corpus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-corpus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-corpus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus-Fundus Membrane Lysate

XBL-11040 0.1 mg
EUR 619.8
Description: Human uterus-fundus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-fundus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-fundus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-fundus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Guinea Pig Uterus Total RNA

GR-411 0.1mg
EUR 160

Mini Pig Uterus Genomic DNA

GN-411 0.1mg
EUR 210

41253-2 RB UTERUS Y 25 EA*

41253-2 25 EA
EUR 263

Hamster Uterus Paraffin Sections

AP-411 10 slides
EUR 228

MiniPig Uterus Paraffin Sections

NP-411 10 slides
EUR 307

Guinea Pig Uterus Genomic DNA

GG-411 0.1mg
EUR 177

Uterus Tissue Slide (Adenocarcinoma)

11-404-10um 10 um
EUR 241.8

Uterus Tissue Slide (Adenocarcinoma)

11-404-4um 4 um
EUR 216.6

cDNA - Human Tumor Tissue: Uterus

C1235274 40 reactions
EUR 636

Rat Uterus, non-pregnant cDNA

RD-411 30 reactions
EUR 243

Guinea Pig Uterus Total Protein

GT-411 0.5mg
EUR 153

Uterus cancer test tissue array

T093 each
EUR 78
Description: Uterus cancer test tissue array, with normal endometrial tissue as control, including TNM, clinical stage and pathology grade, 2 serial sections, 6 cases/24 cores

Leiomyoma of uterus tissue array

SO806 each
EUR 270
Description: Leiomyoma of uterus tissue array, 80 cases/80 cores

Human Uterus Fetal Tissue Lysate

IHUUTEFSTL100UG each
EUR 1133
Description: Human Uterus Fetal Tissue Lysate

Human Uterus Tumor Tissue Lysate

IHUUTETLT100UG each
EUR 361
Description: Human Uterus Tumor Tissue Lysate

Monkey Rhesus Uterus Genomic DNA

UG-411 0.1mg
EUR 210

Guinea Pig Uterus Frozen Sections

GF-411 10 slides
EUR 240

OORA00440-10U - GUINEA PIG UTERUS

OORA00440-10U 1Each
EUR 80

OORA00440-1EA - GUINEA PIG UTERUS

OORA00440-1EA 1Each
EUR 79

Guinea Pig Uterus Paraffin Sections

GP-411 10 slides
EUR 228

Monkey Cynomolgus Uterus Genomic DNA

KG-411 0.1mg
EUR 210

Human Uterus Lysate Membrane Fraction

IHUUTETLM100UG each
EUR 870
Description: Human Uterus Lysate Membrane Fraction

OCPB00088-100UG - Uterus Tumor Lysate

OCPB00088-100UG 0.1mg
EUR 199

The regeneration of Axon is governed by genes sensitive to injuries induced in both neurons and their surrounding non-neuronal cells. Thus, an experimental configuration for the comparative analysis between the regeneration and non-regenerative conditions is essential to identify ideal objectives for the promotion of regeneration-related genes and understand the mechanisms of Axon regeneration. Here we look at the original research that indicates the key factors governing Axon regeneration, including using the expression profile of comparative genes in various systems.