The OPR gene family in watermelon: Genome-wide identification and expression profiling under hormonal treatments and root-knot nematode infection

The 12-oxo-phytododiance acid reductase (OPR) is an important enzyme in the jasmonic biosynthesis path (JA) and therefore plays a vital role in the defense of plants. However, systematic and comprehensive analysis of OPR genes in watermellar and their roles in defense responses are extremely limited. The physicochemical properties, the phylogenetic tree, the genes structure and the cis actor elements of the water melon OPR genes were analyzed by the bioinformatics and QRT-PCR and RNA-SEQ were applied for Analyze the expression of OPR genes in watermelon. A total of five OPR family genes have been identified in the watermelon, which were unevenly distributed in the four chromosomes. Phylogenetic analysis assigned by OPR members of different species of five-subfamil plants (OPRI-OPRV).

The motif compositions of OPR members have been relatively preserved. Expression analysis using QRT-PCR revealed that Clopr genes, with the exception of Clopr5, have been very expressed in flower and fruits. The ARN-SEQ analysis showed that Clopr genes had different expression models during the flesh and skin development. In addition, clopro genes, in particular Clopr2 and Clopr4, have been significantly regulated by an exogenous ja, a salicylic acid (SA) and ethylene (and) treatments.

In addition, the red light has induced the expression of Clopr2 and ClopR4 in sheets and root roots based on root nodes (RKN) -infected plants of the watermelon, suggesting their involvement in the defense induced by red light against RKN. These results provide a theoretical basis for elucidating the various functions of OPR family genes in the watermelon. These items have described 20 simple nucleotide variants (SNV) in 11 genes, most of them in singular families – The exception being the Otog gene. In addition, we analyzed the pathogenicity of each SNV and compared its allelic frequency with sets of reference data to assess its role in the pathogenesis of the FMD. By recovering gene expression data into these genes of different databases, we could classify them according to their gene expression in neuron or internal tissues.

Profiling of human gene expression identifies the main therapeutic targets of tuberculosis Infection: a systematic network meta-analysis

Tuberculosis (TB) is one of the most deadly diseases since antiquity and remains a global health problem. Thus, it is necessary to develop new approaches for the early detection of tuberculosis and to understand the relationship between host and pathogen. In this study, we analyzed flea data sets and compared the transcriptome profile of the healthy individual with a latent infection (LTBI) and TB active patients (TB) and identified the genes expressed differently (DEGS). Then we performed the meta-analysis of the systematic network of degsences, which identified the seven hub genes most influenced as a potential therapeutic target of tuberculosis disease. These target genes are involved in many biological processes such as cell cycle control, apoptosis, complement signaling, improved signaling of cytokine and chemokine, pro-inflammatory responses and immune responses. host.

In addition, we have also identified 22 deducted genes which are mainly engaged in the induction of the innate immune response, a cellular response to interleukin-6, the inflammatory response, the apoptotic process, phosphorylation I-kappab-phosphorylation, The Jak-State signaling pathway, macrophage activation, cell growth and cellular signaling. The appropriate attention of these inferred genes can open a new horizon to understand the defensive mechanisms of tuberculosis disease. The transcriptome and network profiling approach can improve the understanding of the molecular pathogenesis of tuberculosis infection and having implications for the plan and execution of mRNA expression tools to support early diagnoses and The treatment of Mycobacterium tuberculosis (M.TB).

 The OPR gene family in watermelon: Genome-wide identification and expression profiling under hormonal treatments and root-knot nematode infection
The OPR gene family in watermelon: Genome-wide identification and expression profiling under hormonal treatments and root-knot nematode infection

Complete expression of gene expression of human astrockytes treated with an inducible factor of hepatic encephalopathy, alpha 1-anteticmotripsin

Astrocytes are major glial cells that play a crucial role in brain homeostasis. Anomalies in astrocyte function, such as liver encephalopathy (SE) during acute liver failure, may result in cerebral death after cerebral edema and swelling of the associated astrocyte. Recently, we identified alpha 1-antichymotripsin (ACT) to be a biomarker candidate for him.

The act induces swelling of the astrocyte by increasing the aquaporine 4 (AQP4); However, the causal connection between these proteins is not yet clear. In this study, we used a flea profile to display differentially expressed genes (DEGS) in astrocytes treated with act. We then interpreted the ontology of the genes, the react and the complete resource of the enrichment analyzes of the mammal protein complexes (CORUM) of the DEG identified. The results of these analyzes indicated that the DEGs have been enriched in the activation routes of adenylate cyclase cyclase (AC) -Coupled to G protein coupling receptors (GPCR) and have therefore been involved in the cyclic signaling of Monophosphate of adenosine (camp). These results indicate that the law can act as GS-GPCRS ligand and later on the camp.

Adenovirus Purification Kit

K1459-100 100 Preps
EUR 1037

Adenovirus Purification Kit

K1459-20 20 Preps
EUR 606

Lysosome Purification Kit

K235-50
EUR 501

PureXtract RNA Purification Kit

R6111-050 50 prep
EUR 280

PureXtract RNA Purification Kit

R6111-200 200 prep
EUR 874

ViraBind AAV Purification Kit

VPK-140 10 preps
EUR 711
Description: Purification of adeno-associated virus (AAV) using ultracentrifugation is tedious and time-consuming, and yields can be low. Our ViraBind AAV Purification Kits use a single-step proprietary purification matrix, followed by further purification and concentration with a centrifugal concentrator. The result is a higher viral yield with exceptional purity in a fraction of the time.

Rapid Antibody Purification Kit

AKR-160 10 assays
EUR 519
Description: Cell Biolabs? Rapid Antibody Purification kit is designed for rapid, single-step purification of high-quality IgG from ascites, serum and tissue culture media or hybridoma supernatants.

His-tag Purification Kit

abx299036-4U 4 U
EUR 356

Zenoquick PCR Purification Kit

Z3002-050 50 preps
EUR 219

Zenoquick PCR Purification Kit

Z3002-200 4x50 preps
EUR 631

Adenovirus Mini Purification Kit

K1300-10
EUR 544

Adenovirus Mini Purification Kit

K1300-20
EUR 914

Adenovirus Maxi Purification Kit

K1301-10
EUR 1295

Adenovirus Maxi Purification Kit

K1301-2
EUR 533

Adenovirus Maxi Purification Kit

K1301-4
EUR 860

Lentivirus Mini Purification Kit

K1305-10
EUR 544

Lentivirus Mini Purification Kit

K1305-20
EUR 914

Lentivirus Maxi Purification Kit

K1306-10
EUR 1295

Lentivirus Maxi Purification Kit

K1306-2
EUR 533

Lentivirus Maxi Purification Kit

K1306-4
EUR 860

Retrovirus Mini Purification Kit

K1307-10
EUR 544

Retrovirus Mini Purification Kit

K1307-20
EUR 914

Retrovirus Maxi Purification Kit

K1308-10
EUR 1295

Retrovirus Maxi Purification Kit

K1308-2
EUR 533

Retrovirus Maxi Purification Kit

K1308-4
EUR 860

HCV Mini Purification Kit

K1309-10
EUR 669

HCV Mini Purification Kit

K1309-20
EUR 914

HCV Maxi Purification Kit

K1310-10
EUR 1403

HCV Maxi Purification Kit

K1310-2
EUR 533

HCV Maxi Purification Kit

K1310-4
EUR 903

PhosphoSeek? Phosphoprotein Purification Kit

K1401-2
EUR 512

PhosphoSeek? Phosphoprotein Purification Kit

K1401-6
EUR 958

Sperm DNA Purification Kit

K1465-250 250 Preps
EUR 551

Sperm DNA Purification Kit

K1465-50 50 Preps
EUR 318

Saliva DNA Purification Kit

K1468-250 250 Preps
EUR 551

Saliva DNA Purification Kit

K1468-50 50 Preps
EUR 318

Stool DNA Purification Kit

K1474-250 250 Preps
EUR 601

Stool DNA Purification Kit

K1474-50 50 Preps
EUR 348

Mitochondrial DNA Purification Kit

K389-25
EUR 392

GEL Purification Mini Kit (50prep)

FAGPK-001 50 preps
EUR 120

GEL Purification Mini Kit (200prep)

FAGPK-001-1 200 preps
EUR 169

GEL Purification Mini Kit (300prep)

FAGPK-001-2 300 preps
EUR 189

MicroElute GEL Purification Kit (50prep)

FAMGK-001 50 preps
EUR 126

MicroElute GEL Purification Kit (200prep)

FAMGK-001-1 200 preps
EUR 187

HDL Purification Kit (Ultracentrifugation Free)

STA-607 10 preps
EUR 432
Description: Ultracentrifugation of lipoproteins such as HDL, LDL and VLDL can be tedious and time consuming. The HDL Purification Kit provides comparable purity without the need for an ultracentrifuge. Samples are separated into fractions containing HDL and then further purified using standard table-top centrifugation speeds.

ViraBind AAV Purification Mega Kit

VPK-141 2 preps
EUR 572
Description: Purification of adeno-associated virus (AAV) using ultracentrifugation is tedious and time-consuming, and yields can be low. Our ViraBind AAV Purification Kits use a single-step proprietary purification matrix, followed by further purification and concentration with a centrifugal concentrator. The result is a higher viral yield with exceptional purity in a fraction of the time.

ViraBind AAV Purification Mega Kit

VPK-141-5 10 preps
EUR 2312
Description: Purification of adeno-associated virus (AAV) using ultracentrifugation is tedious and time-consuming, and yields can be low. Our ViraBind AAV Purification Kits use a single-step proprietary purification matrix, followed by further purification and concentration with a centrifugal concentrator. The result is a higher viral yield with exceptional purity in a fraction of the time.

PCR Purification Kit (100 preps)

9K-006-0003 100preps
EUR 224

PCR Purification Kit (200 preps)

9K-006-0004 200preps, 200prep
EUR 311

Zenoquick Genomic DNA Purification Kit

Z2003-200 200 preps
EUR 631

Zenoprep Genomic DNA Purification Kit

Z2004-400 400 preps
EUR 550

Zenoprep Serum DNA Purification Kit

Z2005-000 Adjustable
EUR 479

Zenoquick Viral DNA Purification Kit

Z2009-050 50 preps
EUR 340

Zenoquick Viral DNA Purification Kit

Z2009-200 4x50 preps
EUR 941

Bacterial DNA Purification Kit II

K1457-250 250 Preps
EUR 581

Bacterial DNA Purification Kit II

K1457-50 50 Preps
EUR 318

Lentivirus and Retrovirus Purification Kit

K1458-100 100 Preps
EUR 1037

Lentivirus and Retrovirus Purification Kit

K1458-20 20 Preps
EUR 606

Buccal Swab DNA Purification Kit

K1466-250 250 Preps
EUR 551

Buccal Swab DNA Purification Kit

K1466-50 50 Preps
EUR 318

EZCatch? GST-Spin Purification Kit

K861-10
EUR 240

Protein A Antibody Purification Kit

K974-10
EUR 349

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

AfterTri-RNA Purification Mini Kit (200prep)

FAATR-001-1 200 preps
EUR 259

MicroElute GEL/PCR Purification Kit (50prep)

FAEPK-001 50 preps
EUR 131

MicroElute GEL/PCR Purification Kit (200prep)

FAEPK-001-1 200 preps
EUR 208

MicroElute GEL/PCR Purification Kit (300prep)

FAEPK-001-2 300 preps
EUR 229

GEL/PCR Purification Mini Kit (100prep)

FAGCK-001 100 preps
EUR 129

GEL/PCR Purification Mini Kit (300prep)

FAGCK-001-1 300 preps
EUR 172

LDL/VLDL Purification Kit (Ultracentrifugation Free)

STA-606 10 preps
EUR 432
Description: Ultracentrifugation of lipoproteins such as HDL, LDL and VLDL can be tedious and time consuming. The LDL/VLDL Purification Kit provides comparable purity without the need for an ultracentrifuge. Samples are separated into fractions containing LDL plus VLDL and then further purified using standard table-top centrifugation speeds.

ViraBind AAV Purification Kit, Trial Size

VPK-140-T 2 preps
EUR 380
Description: Purification of adeno-associated virus (AAV) using ultracentrifugation is tedious and time-consuming, and yields can be low. Our ViraBind AAV Purification Kits use a single-step proprietary purification matrix, followed by further purification and concentration with a centrifugal concentrator. The result is a higher viral yield with exceptional purity in a fraction of the time.

Exosome Purification and Detection Kit (Plasma)

abx290026-25tests 25 tests
EUR 648

Exosome Purification and Detection Kit (Urine)

abx290027-25tests 25 tests
EUR 648

Zenoquick Soil Microbe DNA Purification Kit

Z2006-050 50 preps
EUR 479

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 402

SeqPure PCR Purification Kit, 5 ml

Z1510001 5 ml
EUR 304
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

SeqPure PCR Purification Kit, 50 ml

Z1510002 50 ml
EUR 899
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

SeqPure PCR Purification Kit, 500 ml

Z1510003 500 ml
EUR 5936
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

RNA/DNA Purification Kit (Magnetic Bead)

DA0623 32 test/kit Ask for price

Adeno-associated Virus Mini Purification Kit

K1302-10
EUR 669

Adeno-associated Virus Mini Purification Kit

K1302-20
EUR 1077

Adeno-associated Virus Maxi Purification Kit

K1303-10
EUR 1403

Adeno-associated Virus Maxi Purification Kit

K1303-2
EUR 533

Adeno-associated Virus Maxi Purification Kit

K1303-4
EUR 903

PCR DNA extraction and purification kit

K1444-100
EUR 338

Plasmid DNA extraction and purification kit

K1445-100
EUR 370

Viral DNA extraction and purification kit

K1446-100
EUR 479

Gel and PCR DNA Purification kit

K1455-250 250 Preps
EUR 480

Gel and PCR DNA Purification kit

K1455-50 50 Preps
EUR 206

Dried Body Fluids DNA Purification Kit

K1464-250 250 Preps
EUR 551

Dried Body Fluids DNA Purification Kit

K1464-50 50 Preps
EUR 318

Eye, Nose & Swabs gDNA Purification Kit

K1467-250 250 Preps
EUR 551

Eye, Nose & Swabs gDNA Purification Kit

K1467-50 50 Preps
EUR 318

Hair, Nails & Feathers DNA Purification Kit

K1469-250 250 Preps
EUR 551

Hair, Nails & Feathers DNA Purification Kit

K1469-50 50 Preps
EUR 318

Protein G Spin Antibody Purification Kit

K546-10
EUR 419

Protein A IgG Purification Buffer Kit

K6529-3
EUR 218

Protein A Spin Antibody Purification Kit

K942-10
EUR 403

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1110

After Tri-RNA Purification Mini Kit (50prep)

FAATR-001 50 preps
EUR 146

96-well GEL Purification Kit (1 prep)

FAGKE-96001 1 prep
EUR 158

96-well GEL Purification Kit (2 prep)

FAGKE-96002 2 preps
EUR 213

96-well GEL Purification Kit (4 prep)

FAGKE-96004 4 preps
EUR 317

Chicken Egg Yolk IgY antibody purification kit

60100 1 kit
EUR 408

96-Well Plate PCR Products Purification Kit

BS365 5XPlates, 480prep
EUR 250.31

96-Well Plate PCR Products Purification Kit

BS3652 2xPlates, 192prep
EUR 155.16

Exosome Purification and Detection Kit (Cell Culture)

abx290025-25tests 25 tests
EUR 648

Agarose gel DNA extraction and purification kit

K1441-100
EUR 425

Blood genomic DNA extraction and purification kit

K1443-100
EUR 479

ToxOut? Protein A (Sepharose) Antibody Purification Kit

K2503-1
EUR 539

ToxOut? Protein G (Sepharose) Antibody Purification Kit

K2505-1
EUR 539

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1132

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1132

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1132

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1132

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1132

EpiNext DNA Purification HT System

P-1063 480 ml
EUR 6022.55
Description: The best epigenetics products

Genomic DNA Extraction and Purification

EP10012 100 Tests
EUR 505

PCR DNA Extraction and Purification

EP10013 100 Tests
EUR 297

Plasmid DNA Extraction and Purification

EP10014 100 Tests
EUR 336

Virus DNA extraction and purification

EP10015 100 Tests
EUR 505

Mini Spincolumn for DNA Purification

S1910-250 250 column
EUR 178

Mini Spincolumn for DNA Purification

S1910-500 500 column
EUR 259

Mini Spincolumn for DNA Purification

S1910-501 5000 column
EUR 1832

COLD STORAGE / PURIFICATION STOCK SOLUTION

99-677-CM 1 L /pk
EUR 208
Description: Specialty Media; Islet products

Magnetic Beads for DNA Purification

M1502-5 5 ml
EUR 277

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 805

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1132

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 153

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 4941

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 4941

Miniprep Kit For Plasmid DNA Extraction And Purification

MB1005-100Reactions 100 Reactions
EUR 143

Miniprep Kit For Plasmid DNA Extraction And Purification

MB1005-250Reactions 250 Reactions
EUR 259

As the camp is known to regulate AQP4 in astrocytes, these results suggest that the law can increase AQP4 by activating AC GPCRS and therefore constitutes a therapeutic target for which it is acute. Abundant proteins (LEAs) are the products of a large gene family in plants that play key roles in the regulation of growth and development, as well as a variety of stress responses. In our study, 67 members of LEA (BDLEA) were identified in the Brachypodium Distachyon L genome. The analyzes of genes structure, evolutionary relations and protein reasons showed that the BDLEAS belonged to six sub-reflamal. The analyzes of the chromosomal locations and duplication numbers revealed that the 67 BDLeas were distributed on the five chromosomes and 26 BDLeas were identified as duplication event products. The results of the annotation of the ontology of genes (GO) suggested that nearly 60% of the BDLeas could be involved in the response to stress.