The OPR gene family in watermelon: Genome-wide identification and expression profiling under hormonal treatments and root-knot nematode infection

The 12-oxo-phytododiance acid reductase (OPR) is an important enzyme in the jasmonic biosynthesis path (JA) and therefore plays a vital role in the defense of plants. However, systematic and comprehensive analysis of OPR genes in watermellar and their roles in defense responses are extremely limited. The physicochemical properties, the phylogenetic tree, the genes structure and the cis actor elements of the water melon OPR genes were analyzed by the bioinformatics and QRT-PCR and RNA-SEQ were applied for Analyze the expression of OPR genes in watermelon. A total of five OPR family genes have been identified in the watermelon, which were unevenly distributed in the four chromosomes. Phylogenetic analysis assigned by OPR members of different species of five-subfamil plants (OPRI-OPRV).

The motif compositions of OPR members have been relatively preserved. Expression analysis using QRT-PCR revealed that Clopr genes, with the exception of Clopr5, have been very expressed in flower and fruits. The ARN-SEQ analysis showed that Clopr genes had different expression models during the flesh and skin development. In addition, clopro genes, in particular Clopr2 and Clopr4, have been significantly regulated by an exogenous ja, a salicylic acid (SA) and ethylene (and) treatments.

In addition, the red light has induced the expression of Clopr2 and ClopR4 in sheets and root roots based on root nodes (RKN) -infected plants of the watermelon, suggesting their involvement in the defense induced by red light against RKN. These results provide a theoretical basis for elucidating the various functions of OPR family genes in the watermelon. These items have described 20 simple nucleotide variants (SNV) in 11 genes, most of them in singular families – The exception being the Otog gene. In addition, we analyzed the pathogenicity of each SNV and compared its allelic frequency with sets of reference data to assess its role in the pathogenesis of the FMD. By recovering gene expression data into these genes of different databases, we could classify them according to their gene expression in neuron or internal tissues.

Profiling of human gene expression identifies the main therapeutic targets of tuberculosis Infection: a systematic network meta-analysis

Tuberculosis (TB) is one of the most deadly diseases since antiquity and remains a global health problem. Thus, it is necessary to develop new approaches for the early detection of tuberculosis and to understand the relationship between host and pathogen. In this study, we analyzed flea data sets and compared the transcriptome profile of the healthy individual with a latent infection (LTBI) and TB active patients (TB) and identified the genes expressed differently (DEGS). Then we performed the meta-analysis of the systematic network of degsences, which identified the seven hub genes most influenced as a potential therapeutic target of tuberculosis disease. These target genes are involved in many biological processes such as cell cycle control, apoptosis, complement signaling, improved signaling of cytokine and chemokine, pro-inflammatory responses and immune responses. host.

In addition, we have also identified 22 deducted genes which are mainly engaged in the induction of the innate immune response, a cellular response to interleukin-6, the inflammatory response, the apoptotic process, phosphorylation I-kappab-phosphorylation, The Jak-State signaling pathway, macrophage activation, cell growth and cellular signaling. The appropriate attention of these inferred genes can open a new horizon to understand the defensive mechanisms of tuberculosis disease. The transcriptome and network profiling approach can improve the understanding of the molecular pathogenesis of tuberculosis infection and having implications for the plan and execution of mRNA expression tools to support early diagnoses and The treatment of Mycobacterium tuberculosis (M.TB).

 The OPR gene family in watermelon: Genome-wide identification and expression profiling under hormonal treatments and root-knot nematode infection
The OPR gene family in watermelon: Genome-wide identification and expression profiling under hormonal treatments and root-knot nematode infection

Complete expression of gene expression of human astrockytes treated with an inducible factor of hepatic encephalopathy, alpha 1-anteticmotripsin

Astrocytes are major glial cells that play a crucial role in brain homeostasis. Anomalies in astrocyte function, such as liver encephalopathy (SE) during acute liver failure, may result in cerebral death after cerebral edema and swelling of the associated astrocyte. Recently, we identified alpha 1-antichymotripsin (ACT) to be a biomarker candidate for him.

The act induces swelling of the astrocyte by increasing the aquaporine 4 (AQP4); However, the causal connection between these proteins is not yet clear. In this study, we used a flea profile to display differentially expressed genes (DEGS) in astrocytes treated with act. We then interpreted the ontology of the genes, the react and the complete resource of the enrichment analyzes of the mammal protein complexes (CORUM) of the DEG identified. The results of these analyzes indicated that the DEGs have been enriched in the activation routes of adenylate cyclase cyclase (AC) -Coupled to G protein coupling receptors (GPCR) and have therefore been involved in the cyclic signaling of Monophosphate of adenosine (camp). These results indicate that the law can act as GS-GPCRS ligand and later on the camp.

MagaBio PCR Purification Kit

TRI-C42S1 50T
EUR 189

MagaBio PCR Purification Kit

TRI-C42S1C 32T
EUR 161.28

MagaBio PCR Purification Kit

TRI-C42S1E 32T
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MagaBio PCR Purification Kit

TRI-C42S1S 32T
EUR 403.2

PCR Purification Kit

20-abx098084
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  • 200 rxns
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RNA Purification Kit

abx098095-25rxns 25 rxns
EUR 276

RNA Purification Kit

MB1006-250preps 250 preps
EUR 476.4

RNA Purification Kit

MB1006-50preps 50 preps
EUR 199.2

PCR Purification Kit

abx098084-100l 100 µl
EUR 250

PCR Purification Kit

abx098084-1ml 1 ml Ask for price

PCR Purification Kit

abx098084-200l 200 µl
EUR 337.5

RNA Purification Kit

abx098095-100l 100 µl
EUR 300

RNA Purification Kit

abx098095-1ml 1 ml Ask for price

RNA Purification Kit

abx098095-200l 200 µl Ask for price

PCR Purification Kit

MBS8808200-200Tests 200Tests
EUR 265

PCR Purification Kit

MBS8808200-50Tests 50Tests
EUR 180

DNA Purification Kit

MBS8808201-200Tests 200Tests
EUR 265

DNA Purification Kit

MBS8808201-50Tests 50Tests
EUR 180

PCR Purification Kit

MBS308017-250Preps 250Preps
EUR 360

PCR Purification Kit

MBS308017-50Preps 50Preps
EUR 190

MagaBio plasmid DNA Purification Kit

TRI-C41M1 100T
EUR 453.6

MagaBio plasmid DNA Purification Kit

TRI-C41S1 50T
EUR 252

MagaBio plasmid DNA Purification Kit

TRI-C41S1C 32T
EUR 214.2

MagaBio plasmid DNA Purification Kit

TRI-C41S1S 32T
EUR 262.08

Lysosome Purification Kit

K235-50 each
EUR 601.2

Adenovirus Purification Kit

K1459-100 100 Preps
EUR 1244.4

Adenovirus Purification Kit

K1459-20 20 Preps
EUR 727.2

Adenovirus Purification Kit

MBS169570-10Preps 10Preps
EUR 815

Adenovirus Purification Kit

MBS169570-5x10Preps 5x10Preps
EUR 3760

Gel DNA Purification Kit

EP006-200T 200T
EUR 93.5

Gel DNA Purification Kit

EP006-50T 50T
EUR 29

RNA/DNA Purification Kit (Magnetic Bead)

DA0623 32 test/kit Ask for price

PURY PCR Purification Kit

P2004-100 100 Prep
EUR 149

PURY Gel Purification Kit

P2005-100 100 Prep
EUR 149

PURY PCR Purification Kit

P2071-100 100 Prep Ask for price

HCV Mini Purification Kit

K1309-20 each
EUR 1096.8

HCV Maxi Purification Kit

K1310-10 each
EUR 1683.6

HCV Maxi Purification Kit

K1310-2 each
EUR 639.6

HCV Maxi Purification Kit

K1310-4 each
EUR 1083.6

MagaBio plus Virus DNA Purification Kit

TRI-C11L1E 96T
EUR 564.48

MagaBio plus Virus DNA Purification Kit

TRI-C11M1B 100T
EUR 478.8

MagaBio plus Virus DNA Purification Kit

TRI-C11S1B 50T
EUR 252

MagaBio plus Virus DNA Purification Kit

TRI-C11S1C 32T
EUR 201.6

MagaBio plus Virus DNA Purification Kit

TRI-C11S1E 32T
EUR 201.6

MagaBio plus Virus DNA Purification Kit

TRI-C11S1S 32T
EUR 262.08

MagaBio plus Total RNA Purification Kit

TRI-C53L1E 96T
EUR 2494.8

MagaBio plus Total RNA Purification Kit

TRI-C53M1B 100T
EUR 1078.56

MagaBio plus Total RNA Purification Kit

TRI-C53M1E 48T
EUR 1340.64

MagaBio plus Total RNA Purification Kit

TRI-C53S1B 50T
EUR 579.6

MagaBio plus Total RNA Purification Kit

TRI-C53S1C 32T
EUR 831.6

MagaBio plus Total RNA Purification Kit

TRI-C53S1E 32T
EUR 831.6

MagaBio plus Total RNA Purification Kit

TRI-C53S1S 32T
EUR 1073.52

MagaBio plus Virus RNA Purification Kit

TRI-C58L1E 32T
EUR 1033.2

MagaBio plus Virus RNA Purification Kit

TRI-C58M1B 100T
EUR 869.4

MagaBio plus Virus RNA Purification Kit

TRI-C58S1B 50T
EUR 453.6

MagaBio plus Virus RNA Purification Kit

TRI-C58S1C 32T
EUR 372.96

MagaBio plus Virus RNA Purification Kit

TRI-C58S1E 32T
EUR 372.96

His-tag Purification Kit

abx299036-4U 4 U
EUR 427.2

Sperm DNA Purification Kit

K1465-250 250 Preps
EUR 661.2

Sperm DNA Purification Kit

K1465-50 50 Preps
EUR 381.6

Stool DNA Purification Kit

K1474-250 250 Preps
EUR 721.2

Stool DNA Purification Kit

K1474-50 50 Preps
EUR 417.6

MagaBio FFPE Tissue RNA Purification Kit

TRI-C36L1E 96T
EUR 1774.08

MagaBio FFPE Tissue RNA Purification Kit

TRI-C36M1 100T
EUR 1345.68

MagaBio FFPE Tissue RNA Purification Kit

TRI-C36M1E 48T
EUR 1108.8

MagaBio FFPE Tissue RNA Purification Kit

TRI-C36S1 50T
EUR 768.6

MagaBio FFPE Tissue RNA Purification Kit

TRI-C36S1E 32T
EUR 592.2

MagaBio FFPE Tissue RNA Purification Kit

TRI-C36S1S 32T
EUR 801.36

MagaBio FFPE Tissue RNA Purification Kit

TRI-C36T1E 16T
EUR 370.44

PCR Circle Purification Kit

EP005-200T 200T
EUR 93.5

PCR Circle Purification Kit

EP005-50T 50T
EUR 29

Saliva DNA Purification Kit

K1468-250 250 Preps
EUR 661.2

Saliva DNA Purification Kit

K1468-50 50 Preps
EUR 381.6

MagaBio Soil Genomic DNA Purification Kit

TRI-C48S1 50T
EUR 415.8

MagaBio Bacterium DNA Purification Kit Ⅲ

TRI-C96L1E 96T
EUR 461.16

MagaBio Bacterium DNA Purification Kit Ⅲ

TRI-C96M1B 100T
EUR 630

MagaBio Bacterium DNA Purification Kit Ⅲ

TRI-C96M1E 48T
EUR 461.16

MagaBio Bacterium DNA Purification Kit Ⅲ

TRI-C96M1S 48T
EUR 456.12

MagaBio Bacterium DNA Purification Kit Ⅲ

TRI-C96S1B 50T
EUR 342.72

MagaBio Bacterium DNA Purification Kit Ⅲ

TRI-C96S1E 32T
EUR 236.88

MagaBio Bacterium DNA Purification Kit Ⅲ

TRI-C96S1S 32T
EUR 262.08

MagaBio Bacterium DNA Purification Kit Ⅲ

TRI-C96T1E 16T
EUR 141.12

Phosphoprotein Purification Kit

AKR-105 2 preps
EUR 358.8
Description: The Phosphoprotein Purification Kit provides an efficient system for quick purification/enrichment of phosphoproteins from various samples. Phosphorylated proteins are affinity purified from lysates with a single-step purification matrix. The entire procedure takes about 4 hours. Each preparation can handle 2.5 mg of total lysate protein (approx. one confluent 100 mm dish).

Phosphoprotein Purification Kit

AKR-106 5 preps
EUR 582
Description: The Phosphoprotein Purification Kit provides an efficient system for quick purification/enrichment of phosphoproteins from various samples. Phosphorylated proteins are affinity purified from lysates with a single-step purification matrix. The entire procedure takes about 4 hours. Each preparation can handle 2.5 mg of total lysate protein (approx. one confluent 100 mm dish).

Max Plasmid Purification Kit

EP003-10T 10T
EUR 80

Max Plasmid Purification Kit

EP003-20T 20T
EUR 119.5

Max Plasmid Purification Kit

MBS8808198-10Tests 10Tests
EUR 250

Max Plasmid Purification Kit

MBS8808198-20Tests 20Tests
EUR 295

Biospin PCR Purification Kit

TRI-C03M1 100T
EUR 141.12

Biospin PCR Purification Kit

TRI-C03S1 50T
EUR 95.76

EcoSpin Gel Purification Kit

EcoGE-50x 50 rxn
EUR 38.5
Description: EcoSpin Gel Purification Kit is designed for effective and fast purification of polymerase chain reaction (PCR) products. Using this kit, primer dimers, free nucleotides in the reaction, salts, and Taq polymerase can be easily and effectively removed.

EcoSpin PCR Purification Kit

EcoPP-50x 50 rxn
EUR 38.5
Description: EcoSpin PCR Purification Kit is designed for effective and fast purification of polymerase chain reaction (PCR) products. Using this kit, primer dimers, free nucleaotides in the reaction, salts, and Taq polymerase can be easily removed. This kit is also suitable for purification of nucleic acids from reactions including restriction digestion, alkaline phosphatase treatment, or kinase reactions.

PCR Purification Kit (100 preps)

9K-006-0003 100preps
EUR 268.8

PCR Purification Kit (200 preps)

9K-006-0004 200preps, 200prep
EUR 373.2

HiPurA® Viral RNA Purification Kit (Magn

MB615M-100PR 1 unit
EUR 255.11
Description: HiPurA® Viral RNA Purification Kit (Magn

HiPurA® Viral RNA Purification Kit (Magn

MB615M-250PR 1 unit
EUR 598.73
Description: HiPurA® Viral RNA Purification Kit (Magn

HiPurA® Viral RNA Purification Kit (Magn

MB615M-480PR 1 unit
EUR 1114.95
Description: HiPurA® Viral RNA Purification Kit (Magn

MagaBio Feces Genomic DNA Purification Kit

TRI-C107S1C-C 32T
EUR 385.56

MagaBio Swabs Genomic DNA Purification Kit

TRI-C35M1 100T
EUR 579.6

MagaBio Swabs Genomic DNA Purification Kit

TRI-C35S1 50T
EUR 317.52

MagaBio Swabs Genomic DNA Purification Kit

TRI-C35S1C 32T
EUR 246.96

MagaBio Swabs Genomic DNA Purification Kit

TRI-C35S1E 32T
EUR 246.96

MagaBio Swabs Genomic DNA Purification Kit

TRI-C35S1S 32T
EUR 488.88

MagaBio pathogens DNA/RNA Purification Kit

TRI-C75L1E 96T
EUR 1008

MagaBio pathogens DNA/RNA Purification Kit

TRI-C75M1 100T
EUR 730.8

MagaBio pathogens DNA/RNA Purification Kit

TRI-C75S1 50T
EUR 390.6

MagaBio pathogens DNA/RNA Purification Kit

TRI-C75S1E 32T
EUR 420.84

MagaBio pathogens DNA/RNA Purification Kit

TRI-C75S1S 32T
EUR 468.72

PCR Purification Kit - Column Kit

PP-201L 250preparations
EUR 251.2

PCR Purification Kit - Column Kit

PP-201S 50preparations
EUR 64.9

MagaBio plus Virus DNA Purification Kit II

TRI-C50S1E 32T
EUR 241.92

MagaBio plus Total RNA Purification Kit II

TRI-C69L1E 96 T
EUR 1512

MagaBio plus Total RNA Purification Kit II

TRI-C69M1B 100T
EUR 1078.56

MagaBio plus Total RNA Purification Kit II

TRI-C69M1E 48 T
EUR 851.76

MagaBio plus Total RNA Purification Kit II

TRI-C69S1B 50T
EUR 579.6

MagaBio plus Total RNA Purification Kit II

TRI-C69S1C 32T
EUR 831.6

MagaBio plus Total RNA Purification Kit II

TRI-C69S1C-C 32T
EUR 504

MagaBio plus Total RNA Purification Kit II

TRI-C69S1E 32T
EUR 831.6

MagaBio plus Total RNA Purification Kit II

TRI-C69S1S 32T
EUR 929.88

MagaBio plus Virus RNA Purification Kit II

TRI-C87L1E 96T
EUR 1209.6

MagaBio plus Virus RNA Purification Kit II

TRI-C87L1E-T 96T
EUR 1209.6

MagaBio plus Virus RNA Purification Kit II

TRI-C87M1B 100T
EUR 869.4

MagaBio plus Virus RNA Purification Kit II

TRI-C87M1E 48T
EUR 650.16

MagaBio plus Virus RNA Purification Kit II

TRI-C87M1E-T 48T
EUR 650.16

MagaBio plus Virus RNA Purification Kit II

TRI-C87M1S 48T
EUR 687.96

MagaBio plus Virus RNA Purification Kit II

TRI-C87S1B 50T
EUR 453.6

MagaBio plus Virus RNA Purification Kit II

TRI-C87S1E 32T
EUR 403.2

MagaBio plus Virus RNA Purification Kit II

TRI-C87S1P 32T
EUR 430.92

MagaBio plus Virus RNA Purification Kit II

TRI-C87S1S 32T
EUR 458.64

MagaBio plus Virus RNA Purification Kit II

TRI-C87T1E 16T
EUR 219.24

MagaBio plus Virus RNA Purification Kit II

TRI-C87T1P 16T
EUR 224.28

MagaBio Insect Genomic DNA Purification Kit

TRI-C33L1E 96T
EUR 700.56

MagaBio Insect Genomic DNA Purification Kit

TRI-C33M1 100T
EUR 579.6

MagaBio Insect Genomic DNA Purification Kit

TRI-C33M1E 48T
EUR 420.84

MagaBio Insect Genomic DNA Purification Kit

TRI-C33S1 50T
EUR 302.4

MagaBio Insect Genomic DNA Purification Kit

TRI-C33S1C-C 32T
EUR 262.08

MagaBio Insect Genomic DNA Purification Kit

TRI-C33S1E 32T
EUR 262.08

MagaBio Insect Genomic DNA Purification Kit

TRI-C33S1S 32T
EUR 441

MagaBio Bacterium DNA Fast Purification Kit

TRI-C45L1E 96T
EUR 743.4

MagaBio Bacterium DNA Fast Purification Kit

TRI-C45M1 100T
EUR 579.6

MagaBio Bacterium DNA Fast Purification Kit

TRI-C45S1 50T
EUR 307.44

MagaBio Bacterium DNA Fast Purification Kit

TRI-C45S1C 32T
EUR 246.96

MagaBio Bacterium DNA Fast Purification Kit

TRI-C45S1E 32T
EUR 246.96

As the camp is known to regulate AQP4 in astrocytes, these results suggest that the law can increase AQP4 by activating AC GPCRS and therefore constitutes a therapeutic target for which it is acute. Abundant proteins (LEAs) are the products of a large gene family in plants that play key roles in the regulation of growth and development, as well as a variety of stress responses. In our study, 67 members of LEA (BDLEA) were identified in the Brachypodium Distachyon L genome. The analyzes of genes structure, evolutionary relations and protein reasons showed that the BDLEAS belonged to six sub-reflamal. The analyzes of the chromosomal locations and duplication numbers revealed that the 67 BDLeas were distributed on the five chromosomes and 26 BDLeas were identified as duplication event products. The results of the annotation of the ontology of genes (GO) suggested that nearly 60% of the BDLeas could be involved in the response to stress.