The OPR gene family in watermelon: Genome-wide identification and expression profiling under hormonal treatments and root-knot nematode infection
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The OPR gene family in watermelon: Genome-wide identification and expression profiling under hormonal treatments and root-knot nematode infection
The 12-oxo-phytododiance acid reductase (OPR) is an important enzyme in the jasmonic biosynthesis path (JA) and therefore plays a vital role in the defense of plants. However, systematic and comprehensive analysis of OPR genes in watermellar and their roles in defense responses are extremely limited. The physicochemical properties, the phylogenetic tree, the genes structure and the cis actor elements of the water melon OPR genes were analyzed by the bioinformatics and QRT-PCR and RNA-SEQ were applied for Analyze the expression of OPR genes in watermelon. A total of five OPR family genes have been identified in the watermelon, which were unevenly distributed in the four chromosomes. Phylogenetic analysis assigned by OPR members of different species of five-subfamil plants (OPRI-OPRV).
The motif compositions of OPR members have been relatively preserved. Expression analysis using QRT-PCR revealed that Clopr genes, with the exception of Clopr5, have been very expressed in flower and fruits. The ARN-SEQ analysis showed that Clopr genes had different expression models during the flesh and skin development. In addition, clopro genes, in particular Clopr2 and Clopr4, have been significantly regulated by an exogenous ja, a salicylic acid (SA) and ethylene (and) treatments.
In addition, the red light has induced the expression of Clopr2 and ClopR4 in sheets and root roots based on root nodes (RKN) -infected plants of the watermelon, suggesting their involvement in the defense induced by red light against RKN. These results provide a theoretical basis for elucidating the various functions of OPR family genes in the watermelon. These items have described 20 simple nucleotide variants (SNV) in 11 genes, most of them in singular families – The exception being the Otog gene. In addition, we analyzed the pathogenicity of each SNV and compared its allelic frequency with sets of reference data to assess its role in the pathogenesis of the FMD. By recovering gene expression data into these genes of different databases, we could classify them according to their gene expression in neuron or internal tissues.
Profiling of human gene expression identifies the main therapeutic targets of tuberculosis Infection: a systematic network meta-analysis
Tuberculosis (TB) is one of the most deadly diseases since antiquity and remains a global health problem. Thus, it is necessary to develop new approaches for the early detection of tuberculosis and to understand the relationship between host and pathogen. In this study, we analyzed flea data sets and compared the transcriptome profile of the healthy individual with a latent infection (LTBI) and TB active patients (TB) and identified the genes expressed differently (DEGS). Then we performed the meta-analysis of the systematic network of degsences, which identified the seven hub genes most influenced as a potential therapeutic target of tuberculosis disease. These target genes are involved in many biological processes such as cell cycle control, apoptosis, complement signaling, improved signaling of cytokine and chemokine, pro-inflammatory responses and immune responses. host.
In addition, we have also identified 22 deducted genes which are mainly engaged in the induction of the innate immune response, a cellular response to interleukin-6, the inflammatory response, the apoptotic process, phosphorylation I-kappab-phosphorylation, The Jak-State signaling pathway, macrophage activation, cell growth and cellular signaling. The appropriate attention of these inferred genes can open a new horizon to understand the defensive mechanisms of tuberculosis disease. The transcriptome and network profiling approach can improve the understanding of the molecular pathogenesis of tuberculosis infection and having implications for the plan and execution of mRNA expression tools to support early diagnoses and The treatment of Mycobacterium tuberculosis (M.TB).
The OPR gene family in watermelon: Genome-wide identification and expression profiling under hormonal treatments and root-knot nematode infection
Complete expression of gene expression of human astrockytes treated with an inducible factor of hepatic encephalopathy, alpha 1-anteticmotripsin
Astrocytes are major glial cells that play a crucial role in brain homeostasis. Anomalies in astrocyte function, such as liver encephalopathy (SE) during acute liver failure, may result in cerebral death after cerebral edema and swelling of the associated astrocyte. Recently, we identified alpha 1-antichymotripsin (ACT) to be a biomarker candidate for him.
The act induces swelling of the astrocyte by increasing the aquaporine 4 (AQP4); However, the causal connection between these proteins is not yet clear. In this study, we used a flea profile to display differentially expressed genes (DEGS) in astrocytes treated with act. We then interpreted the ontology of the genes, the react and the complete resource of the enrichment analyzes of the mammal protein complexes (CORUM) of the DEG identified. The results of these analyzes indicated that the DEGs have been enriched in the activation routes of adenylate cyclase cyclase (AC) -Coupled to G protein coupling receptors (GPCR) and have therefore been involved in the cyclic signaling of Monophosphate of adenosine (camp). These results indicate that the law can act as GS-GPCRS ligand and later on the camp.
Description: The Phosphoprotein Purification Kit provides an efficient system for quick purification/enrichment of phosphoproteins from various samples. Phosphorylated proteins are affinity purified from lysates with a single-step purification matrix. The entire procedure takes about 4 hours. Each preparation can handle 2.5 mg of total lysate protein (approx. one confluent 100 mm dish).
Description: The Phosphoprotein Purification Kit provides an efficient system for quick purification/enrichment of phosphoproteins from various samples. Phosphorylated proteins are affinity purified from lysates with a single-step purification matrix. The entire procedure takes about 4 hours. Each preparation can handle 2.5 mg of total lysate protein (approx. one confluent 100 mm dish).
Description: EcoSpin Gel Purification Kit is designed for effective and fast purification of polymerase chain reaction (PCR) products. Using this kit, primer dimers, free nucleotides in the reaction, salts, and Taq polymerase can be easily and effectively removed.
Description: EcoSpin PCR Purification Kit is designed for effective and fast purification of polymerase chain reaction (PCR) products. Using this kit, primer dimers, free nucleaotides in the reaction, salts, and Taq polymerase can be easily removed. This kit is also suitable for purification of nucleic acids from reactions including restriction digestion, alkaline phosphatase treatment, or kinase reactions.
As the camp is known to regulate AQP4 in astrocytes, these results suggest that the law can increase AQP4 by activating AC GPCRS and therefore constitutes a therapeutic target for which it is acute. Abundant proteins (LEAs) are the products of a large gene family in plants that play key roles in the regulation of growth and development, as well as a variety of stress responses. In our study, 67 members of LEA (BDLEA) were identified in the Brachypodium Distachyon L genome. The analyzes of genes structure, evolutionary relations and protein reasons showed that the BDLEAS belonged to six sub-reflamal. The analyzes of the chromosomal locations and duplication numbers revealed that the 67 BDLeas were distributed on the five chromosomes and 26 BDLeas were identified as duplication event products. The results of the annotation of the ontology of genes (GO) suggested that nearly 60% of the BDLeas could be involved in the response to stress.
