Gene expression profiling in dorsolateral prostates of prepubertal and adult Sprague-Dawley rats dosed with estradiol benzoate, estradiol, and testosterone
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Gene expression profiling in dorsolateral prostates of prepubertal and adult Sprague-Dawley rats dosed with estradiol benzoate, estradiol, and testosterone
The imbalance of testosterone to estradiol ratio has been associated with the development of prostate disease. Although mouse models of prostate disease caused by endocrine-disruptive chemicals (EDC) and / or exposure to the hormone that is used to analyze the gene expression profiles in prostate, most studies utilize a single endpoint. In this study, microarray analysis is used to profile gene expression in the prostate tissue of mice after exposure to EDCs and sex hormones during some point in time (before puberty to adulthood).
We use the network dorsolateral prostate of Sprague-Dawley rats (male offspring) and postnatal given estradiol benzoate (EB) on days postnatal (PND) 1, 3, and 5, followed by treatment with growth hormones [estradiol (E) and testosterone (T)] on the PND 90-200, as described by Ho et al. microarray analysis performed to profile gene expression in dorsolateral prostate, and the results are validated by qRT-PCR. Gene interactions receptors cytokines, cell adhesion molecules and chemokines are upregulated in the group EB + T + E on PND 145 and 200.
In addition, the initial stage downregulation of anti-inflammatory genes: bone morphogenetic protein 7 gene was observed. These findings indicate that exposure to EB, T, and E activates multiple pathways and simultaneously downregulates anti-inflammatory genes. Interestingly, these genes are reportedly expressed in prostate cancer tissue line / cell. Further research is needed to clarify the mechanisms, including analysis using human prostate tissue.
Gene expression profiling in dorsolateral prostates of prepubertal and adult Sprague-Dawley rats dosed with estradiol benzoate, estradiol, and testosterone
De Novo Assembly transcriptome and Gene Expression Profiling of copepods Calanus helgolandicus Feeding the PUA-Producing marinoi Diatom Skeletonema
Diatoms are the dominant component of marine phytoplankton. Some diatoms produce secondary metabolites, ie oxylipins, with teratogenic effects on their main predator, the copepod crustaceans. Our study reports assembled de novo transcriptome of copepod Calanus calanoid helgolandicus eat in oxylipin-producing diatoms Skeletonema marinoi. Differential expression analysis was also conducted among women exposed diatoms copepods and minimum Prorocentrum whack control, which does not produce oxylipins.
Our results showed that the transcripts are involved in carbohydrate, amino acids, folic acid and methionine metabolism, embryogenesis, and response to stimuli that differentially expressed in the two conditions. The expression of 27 genes that have belonged functional categories were also analyzed by RT-qPCR in C. helgolandicus females exposed to the admixture of oxylipins heptadienal and octadienal at a concentration of 10 pM, 15 pM, and 20 pM.
The results confirmed the differential expression analysis, with the up-regulation of genes involved in stress response and down-regulation of genes related to folate and methionine metabolism, embryogenesis, and signals. Overall, we offer new insight into the mechanism of action of oxylipins on maternal-induced embryo abnormalities. Our results can also help identify gene biomarkers associated with reproductive failure associated diatoms on natural copepod populations in the sea.
Rising demand for commercial picrosides and overexploitation of Picrorhiza kurroa of natural habitat has to start alternative strategy for the sustainable production of metabolites. In this study, wild leaf explants P. kurroa used to produce callus was friable under different culture conditions, ie, dark and bright with two variants temperature (15 ° C and 25 ° C). Afterwards, callus cell lines screened based on biomass growth and accumulation of metabolites content.
Description: MPYS Antibody: MPYS is a recently identified plasma membrane tetraspanner that is associated with major histocompatibility complex class II (MHC-II) and mediates its transduction of apoptotic signals. It has also been found to be associated with VISA, a mitochondrial protein that acts as an adaptor in virus-triggered signaling. MPYS also interacts with IRF3 and recruits the kinase TBK1 to the VISA-associated complex, acting as a critical mediator of virus-triggered IRF3 activation and interferon (IFN) expression. It is thought that the binding of nucleic acid to the innate immune protein RIG-I causes complex formation between RIG-I, VISA, and MPYS. This complex then recruits TBK1 to phosphorylate IRF3 which then directly activates IFN transcription. At least three isoforms of MPYS are known to exist.
Results showed that maximum callus growth index along with the antioxidant potential (IC50-40.88 ug / mL) and total phenol content (41.35 mg / mg) was observed under the dark 25 ° C.
